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In another embodiment, the present invention is directed toward a modified TetR(A) protein comprising an amino acid substitution at a position selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 99, 100, 101, 103, 114, 127, 158, 159, 160, 179, 193, 197, and 201 of the TetR(A) protein as depicted in SEQ ID NO: 34, wherein said modified TetR(A) protein binds a TetR(A) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline; wherein: the amino acid substitution at position 59 is asparagine; the amino acid substitution at position 70 is valine; the amino acid substitution at position 71 is valine; the amino acid substitution at position 91 is glutamine; the amino acid substitution at position 95 is selected from the group consisting of glycine and glutamic acid; the amino acid substitution at position 95 is glycine; the amino acid substitution at position 95 is glutamic acid; the amino acid substitution at position 96 is arginine; the amino acid substitution at position 96 is glutamic acid; the amino acid substitution at position 99 is glutamic acid; the amino acid substitution at position 100 is alanine; the amino acid substitution at position 101 is histidine; the amino acid substitution at position 103 is serine; the amino acid substitution at position 114 is valine; the amino acid substitution at position 127 is selected from the group consisting of arginine, lysine, and histidine; the amino acid substitution at position 158 is cysteine; the amino acid substitution at position 159 is leucine; the amino acid substitution at position 160 is valine; the amino acid substitution at position 179 is valine; the amino acid substitution at position 193 is glycine; the amino acid substitution at position 197 is tryptophan; and the amino acid substitution at position 201 is histidine? 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WHO WE ARE
South Capitol Bridgebuilders is the design-build contractor for the New Frederick Douglass Memorial Bridge. South Capitol Bridgebuilders is a joint venture between Archer Western Construction LLC (Walsh Group) and Granite Construction Co. with AECOM serving as the bridge designer. South Capitol Bridgebuilders is committed to meeting our local hiring goals and Disadvantaged Business Enterprises (DBE) contracting goals.
The New Frederick Douglass Memorial Bridge Project will provide on-the-job training opportunities aimed at developing individuals
The New Frederick Douglass Memorial Bridge Project has employment opportunities in design and engineering
Click and watch the video below to see a glimpse of the project